| 藻蓝蛋白原位还原Ag（Ⅰ）与纳米Ag（O）形成动态过程的谱学研究 |
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| 引用本文： | 杨芳,郭振江,白燕,郑文杰.藻蓝蛋白原位还原Ag（Ⅰ）与纳米Ag（O）形成动态过程的谱学研究[J].光谱学与光谱分析,2007,27(1):23-27. |
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| 作者姓名： | 杨芳 郭振江 白燕 郑文杰 |
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| 作者单位： | [1]暨南大学化学系,广东广州510632 [2]暨南大学水生生物研究所,广东广州510632 |
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| 基金项目： | 广东省自然科学基金重点项目（05103295）和广东省自然科学基金团队项目（039213）资助 |
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| 摘 要： | 在4℃避光条件下，将藻蓝蛋白（简称PC）与AgNO3作用，通过UV，FS，FTIR等谱学方法研究了PC与Ag（Ⅰ）原位还原与纳米Ag（0）粒子形成的动态过程。结果显示：PC在615nm的特征吸收峰强度明显减弱，并随Ag（Ⅰ）浓度增加和时间延长单调降低；PC的荧光发射峰和荧光激发峰也均呈现衰减趋势。同步荧光光谱观察到纳米Ag（0）粒子形成的动态过程。用TEM观察到所形成的Ag（0）分散于PC表面，形成PC为核，纳米银为壳部分包覆的生物缀合物，粒子呈球形，有较窄的分布尺寸，粒径在15～30nm之间。
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| 关 键 词： | 银 钝顶螺旋藻 藻蓝蛋白 光谱 纳米 |
| 文章编号： | 1000-0593（2007）01-0023-05 |
| 收稿时间： | 2005-11-16 |
| 修稿时间： | 2006-03-06 |
Spectra of in situ reduction of Ag( I ) by phycocyanin and dynamic formation of Ag(0)] |
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| YANG Fang, GUO Zhen-jiang , BAI Yan, ZHENG Wen-jie.Spectra of in situ reduction of Ag( I ) by phycocyanin and dynamic formation of Ag(0)][J].Spectroscopy and Spectral Analysis,2007,27(1):23-27. |
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| Authors: | YANG Fang GUO Zhen-jiang BAI Yan ZHENG Wen-jie |
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| Institution: | 1. Department of Chemistry, Jinan University, Guangzhou 510632, China; 2. Institute of Hydrobiology, Jinan University, Guangzhou 510632, China |
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| Abstract: | The interaction of Ag( I ) and Phycocyanin(PC for short) was investigated by ultraviolet-visible, fluorescence and infrared spectra under the condition of 4 degrees C and without light. The results showed that the characteristic absorption of PC at 615 nm decreased gradually with the increase in Ag( I ) concentration and interaction time. Accordingly, its fluorescence emission peak and two fluorescence excitation peaks also decreased. Dynamic formation of nano-Ag(0) was also observed by synchronous fluorescence spectra, showing that Ag(0) was formed by the PC in situ reduction. It was observed by TEM that Ag(0) dispersed in PC, which formed a bioconjugate with PC as the core and nano-Ag(0) as the crust. The bioconjugate was round and its diameter was about 15-30 nm. |
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| Keywords: | Silver Spirulina platensis Phycocyanin Spectroscopy Nanoparticle |
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