Calcium alginate gel as encapsulation matrix for coimmobilized enzyme systems |
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| Authors: | Blandino A Macías M Cantero D |
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| Institution: | (1) Biological and Enzymatic Reactors Research Group, Department of Chemical Engineering, Food Technology and Environmental Technologies, Faculty of Sciences, University of Cádiz (UCA), 11510 Puerto Real (Cádiz), Spain |
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| Abstract: | Encapsulation within calcium alginate gel capsules was used to produce acoimmobilized enzyme system. Glucose oxidase (GOD)
and catalase (CAT) were chosen as model enzymes. The same values of V
max and K
mapp
for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were
compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as
immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the
structure of the capsules. |
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| Keywords: | Calcium alginate gel capsules gel beads coimmobilization glucose oxidase catalase |
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