| 头孢拉定与血清白蛋白相互作用的光谱学研究 |
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| 引用本文： | 宋熙熙,陈树大,刘涛. 头孢拉定与血清白蛋白相互作用的光谱学研究[J]. 化学分析计量, 2011, 20(6): 33-35 |
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| 作者姓名： | 宋熙熙 陈树大 刘涛 |
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| 作者单位： | 嘉兴学院生物与化学工程学院,浙江嘉兴,314001 |
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| 摘 要： | 采用荧光和紫外吸收光谱法研究头孢拉定和牛血清白蛋白(BSA)的相互作用.研究发现,头孢柱定荧光猝灭牛血清白蛋白是由于形成了头孢拉定-牛血清白蛋白复合物.分别计算了不同温度下双分子猝灭常数kq和结合常数K.由热力学参数焓变(△H)、熵变(△S)和吉布斯自由能(△G),推断出头孢拉定与BSA的相互作用是一个疏水作用的自发过...
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| 关 键 词： | 头孢拉定 牛血清白蛋白 荧光猝灭 热力学参数 |
SPECTROSCOPIC STUDIES OF INTERACTION BETWEEN CEPHRADINE AND BOVINE SERUM ALBUMIN |
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| Song Xixi,Chen Shuda,Liu Tao. SPECTROSCOPIC STUDIES OF INTERACTION BETWEEN CEPHRADINE AND BOVINE SERUM ALBUMIN[J]. Chemical Analysis And Meterage, 2011, 20(6): 33-35 |
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| Authors: | Song Xixi Chen Shuda Liu Tao |
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| Affiliation: | (College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China) |
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| Abstract: | The interaction between cephradine and bovine serum albumin （BSA）was investigated by fluorescence and UV absorption spectroscopy. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by cephradine resulted from the formation of cephradine-BSA complex. The quenching constant kq and the binding constant K at different temperatures were calculated. The thermodynamic parameters, enthalpy change（ △H）, entropy change（△S） and Gibbs free energy change （△G） were calculated respectively, which indicated that the interaction of cephradine with BSA was a spontaneous process mainly by hydrophobic interaction. |
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| Keywords: | cephradine bovine serum albumin fluorescence quenching thermodynamic parameter |
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