Spherical molecularly imprinted polymer particles: a promising tool for molecular recognition in capillary electrokinetic separations |
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Authors: | De Boer Theo Mol Roelof De Zeeuw Rokus A De Jong Gerhardus J Sherrington David C Cormack Peter A G Ensing Kees |
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Affiliation: | Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, Groningen, The Netherlands. |
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Abstract: | Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)-ephedrine-imprinted microspheres (100-200 nm) which were polymerized from methacrylic acid and 1,1,1-Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1% w/v suspension in an aqueous 10 mM phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min. |
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