| 毛细管电泳用于评价脱氧核糖核酸退火反应 |
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| 引用本文： | 何新亚,周小棉,林炳承.毛细管电泳用于评价脱氧核糖核酸退火反应[J].分析化学,2003,31(1):10-13. |
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| 作者姓名： | 何新亚 周小棉 林炳承 |
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| 作者单位： | 中国科学院大连化学物理研究所,大连,116023 |
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| 摘 要： | 将毛细管电泳技术用于定性和定量评价互补单链脱氧核糖核酸（DNA）的退火反应，从电泳信号能直接观察退火效率。解释了双链DNA的电泳迁移特性，对DNA产品基质中的NaCl浓度进行了测定，考察了DNA样品中NaCl浓度对进样的稀释效应。在所应用的实验条件下，NaCl浓度在20mmol/L以内稀释效应的影响是可以忽略的。
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| 关 键 词： | 评价 退火反应 毛细管电泳 脱氧核糖核酸 DNA 合成 分析 脱盐 |
Using Capillary Electrophoresis to Evaluate Deoxyribonucleic Acid Annealing Reaction |
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| He Xinya,Zhou Xiaomian,Lin Bingcheng.Using Capillary Electrophoresis to Evaluate Deoxyribonucleic Acid Annealing Reaction[J].Chinese Journal of Analytical Chemistry,2003,31(1):10-13. |
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| Authors: | He Xinya Zhou Xiaomian Lin Bingcheng |
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| Institution: | He Xinya,Zhou Xiaomian,Lin Bingcheng * |
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| Abstract: | Capillary Electrophoresis was used to evaluate the annealing of two complemental single stranded deoxyribonucleic acid (DNA) qualitatively and quantitatively. The annealing efficiency can be observed from the electropherogram. The migration characteristic of the double stranded DNA was explained. The NaCl concentration in the annealing DNA product matrix was determined. In general the existence of NaCl can lead the sample to dilute, but this factor could be neglected when the concentration of NaCl was <20 mmol/L in our experimental conditions. |
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| Keywords: | Capillary electrophoresis deoxyribonucleic acid annealing |
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