Validation of Chiral Electrokinetic Chromatography Methods Using Highly Sulfated Cyclodextrins: Determination of Enantiomeric Purity of Aromatase Inhibitors |
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Authors: | C Danel C Foulon J F Goossens J P Bonte C Vaccher |
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Institution: | (1) Laboratoire de Chimie Analytique EA 4034, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille 2, BP 83, 59006 Lille Cédex, France |
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Abstract: | Electrokinetic chromatography methods for the enantiomeric resolution of four imidazole derivatives, potential aromatase inhibitors,
were developed using highly Sulfated α-, highly Sulfated β- and highly Sulfated γ- CDs as chiral selectors at acidic pH. The optimization of the various operational parameters (nature and concentration of
the CD, capillary length, buffer concentration, presence of organic modifier in the electrolyte, temperature and voltage)
permits to obtain resolution factors superior to 3, for each racemic analyte, with migration times of the second enantiomers
less than 6 minutes. The four optimal analytical methods were validated prior to the determination of the enantiomeric purity
of the eight enantiomers previously isolated and analyzed by chiral HPLC. |
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Keywords: | Capillary electrophoresis Chiral electrokinetic chromatography Aromatase inhibitors Sulfated cyclodextrins Enantiomeric purity |
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