Separation of hyaluronic acid by ultrahigh-voltage capillary gel electrophoresis |
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| Authors: | Hutterer Katariina M Jorgenson James W |
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| Institution: | Department of Chemistry, University of North Carolina at Chapel Hill, NC, USA. |
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| Abstract: | Hyaluronic acid was separated using 95 kV applied potential in a polyacrylamide gel-filled capillary. The results of this separation were compared to those obtained using a capillary electrophoresis instrument operated at a more conventional potential of 15 kV. For lower-molecular-weight oligomers, the separation efficiency was found to improve by about tenfold, and the resolution by about threefold. However, the improvement in resolution declined as the polymer molecular weight increased. |
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| Keywords: | Capillary electrophoresis Carbohydrate Hyaluronic acid Ultrahigh voltage |
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