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Application of SPE for the HPLC analysis of taxanes fromTaxus cell cultures |
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| Authors: | G Theodorídis C F de Jong G Laskaris R Verpoorte |
| Institution: | (1) Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Leiden University, P.O Box 9502, 2300 RA Leiden, The Netherlands;(2) Present address: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 540 06 Thessaloniki, Greece;(3) Present address: Department of Phytopathology, Wageningen Agricultural University, Binnenhaven 9, 6709 PD Wageningen, The Netherlands |
| Abstract: | Summary Solid phase extraction (SPE) methods are studied for the sample pretreatment for HPLC analysis ofTaxus cell suspension cultures. Various types of SPE materials were tested for the extraction of both the taxane standards and samples of various origin. Comparison between the different cartridges and the different elution solvents are made in terms of extraction recovery and sample clean-up. Selective elution of the taxanes is achieved by a gradient elution scheme. |
| Keywords: | Column liquid chromatography Solid Phase Extraction RP-HPLC gradient analysis Taxus Cell Cultures Taxol and Taxanes |
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