An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis |
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| Authors: | Wang Weixue Wang Ke Li Jikun Nellutla Saritha Smirnova Tatyana I Oldfield Eric |
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| Institution: | Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, 607 South Mathews Avenue, Urbana, Illinois 61801, USA. |
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| Abstract: | IspG is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron-nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the hyperfine interactions in a reaction intermediate. These results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand. |
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