| 荧光光谱法测定香椿叶总黄酮的含量 |
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| 引用本文： | 常永芳,冯俊霞,于宏伟,郧海丽. 荧光光谱法测定香椿叶总黄酮的含量[J]. 光谱实验室, 2010, 27(5): 1773-1776 |
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| 作者姓名： | 常永芳 冯俊霞 于宏伟 郧海丽 |
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| 作者单位： | 石家庄学院化工学院,石家庄市高新技术开发区长江大道6号,050035;石家庄学院化工学院,石家庄市高新技术开发区长江大道6号,050035;石家庄学院化工学院,石家庄市高新技术开发区长江大道6号,050035;石家庄学院化工学院,石家庄市高新技术开发区长江大道6号,050035 |
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| 摘 要： | 根据黄酮类化合物能与Al3+形成稳定的荧光络合物,建立一种测定香椿叶总黄酮的新方法。用50%乙醇溶液浸提,65℃超声提取香椿叶中有效成分,以芦丁为标样,在激发波长λex=437nm,发射波长λem=534nm下,测定香椿叶中总黄酮的含量,并进行加标回收实验,验证方法的准确性。方法的检出限为3.25×10-9mol.L-1,线性范围在1.23×10-9—2.62×10-6mol.L-1之间,线性回归方程y=1.862x+0.361,相关系数r=0.9983,平均回收率为100.3%,相对标准偏差(RSD)为0.99%。
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| 关 键 词： | 荧光光谱法 香椿叶 总黄酮 |
Determination of Total Flavone in Leaves of Toona Sinensis by Fluorescence Spectrometry |
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| CHANG Yong-Fang,FENG Jun-Xia,YU Hong-Wei,YUN Hai-Li. Determination of Total Flavone in Leaves of Toona Sinensis by Fluorescence Spectrometry[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(5): 1773-1776 |
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| Authors: | CHANG Yong-Fang FENG Jun-Xia YU Hong-Wei YUN Hai-Li |
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| Affiliation: | CHANG Yong-Fang FENG Jun-Xia Yu Hong-Wei YUN Hai-Li ( College of Chemical Engineering, Shijiazhuang University, Shijiazhuang 0 5 0 0 3 5, P. R. China ) |
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| Abstract: | A new method for determination of total flavone in leaves of toona sinensis was established based on the reaction of total flavone with aluminium ion. The effective components were extracted from leaves of Toona sinensis by ultrasonic at 65℃ with 50% ethanol, total flavone was determined by fluorescence spectrometry with rutin as standard reference,at the excitation wavelength of 437nm and the emission wavelength of 534nm. The detection limit is 3. 25 × 10^-9mol · L^-1 in the linear range of 1. 23 × 10^-9--2. 62× 10^-6 mol · L^-1 with the regression equation of y=1. 862x＋0. 361,correlative coefficient was 0. 9983,average rate of recovery was 100. 3% ,RSD was 0. 99%. |
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| Keywords: | Fluorescence Spectrometry Leaves of Toona Sinensis Total Flavone |
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