Pyrrolo-dC and pyrrolo-C: fluorescent analogs of cytidine and 2-deoxycytidine for the study of oligonucleotides |
| |
Authors: | David A Berry Kee-Yong Jung Anthony D Sercel Hugh Mackie Robert L Somers |
| |
Institution: | a Berry & Associates, Inc., 2434 Bishop Circle East, Dexter, MI 48130, USA b Glen Research Corporation, 22825 Davis Drive, Sterling, VA 20164, USA |
| |
Abstract: | Pyrrolo-dC (1a, 6-methyl-3-(2-deoxy-β-d-ribofuranosyl)-3H-pyrrolo2,3-d]pyrimidin-2-one) and its cyanoethyl phosphoramidite 2a were synthesized. The latter was incorporated into oligodeoxyribonucleotides by standard automated synthesis techniques, where pyrrolo-dC was found to serve as a fluorescent analog of deoxycytidine. The cyanoethylphosphoramidite (2b) of pyrrolo-C (2a, 6-methyl-3-(β-d-ribofuranosyl)-3H-pyrrolo2,3-d]pyrimidin-2-one) was also synthesized and may find use for the site-specific incorporation of a fluorescent cytidine analog into oligoribonucleotides. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|