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KINETIC STUDIES ON CHEMILUMINESCENCE OF CHLOROPLASTS INDUCED BY CHANGES IN ION CONCENTRATION
Authors:H HARDT  S MALKIN
Abstract:Abstract— Acid-base, acid-, and salt-induced chemiluminescences of chloroplasts were investigated in relation to their dependences upon several time parameters:
  • 1 The dependence of the three chemiluminescences on the preillumination time was similar; the luminescences rose to a maximum and then decayed to a steady-state This behaviour depends on the preillumination level and the length of the dark time following the preillumination. Analysis of the above indicates the formation of quenching entities during the preillumination which react with the luminescence precursors mainly in the dark. In contrast, the delayed light measured after 22 msec from the preillumination, showed a much slower but smooth rise to the steady-state with no subsequent drop.
  • 2 During the dark period following preillumination the ability of the system to chemi-luminescence decayed with a first order rate.
  • 3 During emission the above chemiluminescences decayed with a first order law. The total emission in different experiments was also proportional to the maximum emission, in agreement with a first order law of decay.
  • 4 The comparison of the kinetic behaviour of the three types of chemiluminescence indicates common precursors of luminescence for at least the acid-base and the salt types. The delayed emission at 22 msec however, seems to have separate precursors than the three chemiluminescences.
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