首页 | 本学科首页   官方微博 | 高级检索  
     

DECARBOXYLATION DURING THE FORMATION OF THE FLUORESCENT NAD DERIVATIVE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
作者姓名:周筠梅  徐功巧  赵康源  邹承鲁
作者单位:Intitute of Biophysics,Academia Silica,Beijing,Intitute of Biophysics,Academia Silica,Beijing,Intitute of Biophysics,Academia Silica,Beijing,Intitute of Biophysics,Academia Silica,Beijing
摘    要:During the formation of the fluorescent NAD derivative of rabbit muscle D-glyceral-dehydc-3-phosphate dehydrogenase modified with ~(14)C-1-iodoacetate by ultraviolet irradiation about 1/2 of the total radioactivity is removed as CO_2, when the fluorescent intensity has reached a maximum, With a weak light source, it can be shown that the formation of the fluorescent derivative precedes the decarboxylation. For the Bacillus stearothermophilus enzyme the formation of the fluorescent NAD derivative is an all-sites reaction and is accompanied by the removal of 4 CO_2 molecules showing conclusively that CO_2 has come from the subunits carrying the fluorophore.It is known that the yeast enzyme can be carboxymethylated either at 4, or under carefully controlled conditions, at 2 of the active site SH groups. Experiments with ~(14)C-labelled NAD~ show that both the tetrakis and his carboxymethylated enzymes form 2 molecules of the fluorescent NAD derivative. However, for the biscarboxymethylated enzyme, only on

本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号