Fluorescence imaging of multi-component wetting |
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Authors: | Anne M Grillet Carlton F Brooks Benjamin J Ash John A Emerson |
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Institution: | (1) Sandia National Laboratories, P.O. Box 5800, Albuquerque, NM 87185, USA |
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Abstract: | Fluorescence imaging methods are explored as tools to study multi-component wetting of liquids. A novel approach was employed
using polymer labeled with contrasting fluorescent dyes, which allow optical recognition and also caused differences in surface
tension between blend components. Total internal reflection-fluorescence microscopy probes a thin surface layer to the order
of 100 nm to investigate the structure of the spreading drop next to the substrate. Dye segregation out of solution and phase
separation of polymer blends were observed. Confocal microscopy can visualize the larger three-dimensional structure of sessile
drops. Component segregation was observed and quantified using relative fluorescence intensities. |
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