Supramolecular immobilization of glucose oxidase on gold coated with cyclodextrin-modified cysteamine core PAMAM G-4 dendron/Pt nanoparticles for mediatorless biosensor design |
| |
Authors: | Paula Díez Ciprian-George Piuleac Paloma Martínez-Ruiz Santiago Romano María Gamella Reynaldo Villalonga José M Pingarrón |
| |
Institution: | 1. Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain 2. Department of Chemical Engineering, “Gh. Asachi” Technical University, Bd. D. Mangeron No. 71A, 700050, Iasi, Romania 3. Department of Organic Chemistry I, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain
|
| |
Abstract: | Cysteamine core polyamidoamine G-4 dendron branched with β-cyclodextrins was chemisorbed on the surface of Au electrodes and further coated with Pt nanoparticles. Adamantane-modified glucose oxidase was subsequently immobilized on the nanostructured electrode surface by supramolecular association. This enzyme electrode was used to construct a reagentless amperometric biosensor for glucose, making use of the electrochemical oxidation of H2O2 generated in the enzyme reaction. The amperometric response of the biosensor was rapid (6 s) and a linear function of glucose concentration between 5 and 705 μmol?L?1. The biosensor had a low detection limit of 2.0 μmol?L?1, sensitivity of 197 mA?mol?1?L?cm?2, and retained 94 % of its initial response after storage for nine days at 4 °C. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|