| Abstract: | Diastereoisomers of specifically labeled oxytocin derivatives were resolved using reverse phase high pressure liquid chromatography. The peptides 1-hemi-DL-alpha-2H]cystine]oxytocin, 6-hemi-DL-alpha-2H]cystine]oxytocin, 2-DL-alpha-2H]tyrosine]oxytocin and8-DL-2-13C]leucine]oxytocin were readily separated using the conditions described. The diastereoisomers of the oxytocin analog 3-DL-2-13C]leucine]oxytocin also demonstrated baseline resolution under the same conditions. The procedure offers the investigator a rapid method for screening synthetic oxytocin peptides for undesirable diastereoisomeric by-products. |
