Comparison of carboxymethyl-cellulose cation-exchange chromatography and high-performance liquid chromatography in the purification of guinea-pig insulin from pancreatic extracts

Guinea-pig insulin was purified from pancreatic extracts either by carboxymethyl-cellulose cation-exchange chromatography with a sodium chloride gradient or by high-performance liquid chromatography (HPLC) on octadecyl silica with mixtures of acetonitrile and phosphate buffer. HPLC proved to be superior to ion-exchange chromatography in the purification of insulin with respect both to time saving and to the purity of the product.