高效液相色谱法测定我国北方红枣中环磷酸腺苷的含量

采用高效液相色谱（HPLC）法准确测定环磷酸腺苷（cAMP）含量，并对我国北方红枣中的cAMP含量进行测定.以Hibar ODS-C18（250 mm×4.6 mm，5 μm）为色谱柱，流动相为甲醇:2%醋酸（体积比为5:95），流速0.5 mL/min，检测器为Waters 996，检测温度为25℃，检测波长为254 nm.标准曲线在0.1~1.0 mg/mL范围内线性良好，加标回收率为102.1%，相对标准误差为0.63%.同时，采用的条件能将红枣中cAMP与其他成分的峰完全分离，发现不同产地的红枣中cAMP含量不同，新疆地区所产红枣的质量分数最高，能达到372 μg/g.方法可准确测定我国北方红枣中环磷酸腺苷的含量，灵敏度高，可为红枣中cAMP的提取和高值化利用提供重要的技术依据.

Determination of cAMP in Jujube from North of China by High Performance Liquid Chromatography

To determine accurately the content of cyclic adenosine monophosphate (cAMP) by high performance liquid chromatography(HPLC) in jujube in northern China, Hibar ODS-C18 (250 mm×4.6 mm, 5 μm) was used as the chromatographic column, the mobile phase was methanol:2% acetic acid (volume ratio:5:95), the flow rate was 0.5 mL/min, and the detector was a Waters 996. The detection temperature was 25℃ and the detection wavelength was 254 nm. The standard curve was of good linearity in the range of 0.1~1.0 mg/mL. The recovery rate was 102.1% and the relative standard error was 0.63%. The conditions used in this method can completely separate cAMP and other components in jujube. We found that the contents of cAMP in jujube from different producing areas were different, and the content of cAMP in jujube from Xinjiang was the highest and reached 372 μg/g. This method can accurately determine the content of adenosine phosphate in jujube in northern China, with high sensitivity, thus provides an important technical basis for the extraction and high-value utilization of cAMP in jujube.