| Abstract: | A highly effective easily reproducible method of obtaining high purity preparations of glucose isomerase from initial and hybrid strains of Streptomyces atratus has been developed. Two enzymes have been characterized, with molecular masses of 160 and 120 kDa, isoelectric points of 5.2 and 5.0, pH optima of 8.0 and 9.0, and temperature optima of 80 and 90°C, respectively. The activity of glucose isomerase preparations obtained by purification from a hybrid strain of Streptomyces atratus was 60–70% greater than that of analogous preparations from the initial parental strain.Institute of Microbiology of the Academy of Sciences, Republic of Uzbekistan, Tashkent, fax (3712) 417129 Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 903–907, November–December, 1996. Original article submitte March 11, 1996. |
