| 猪瘟病毒全长突变型cDNA克隆的构建及其在细胞水平的恢复 |
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| 引用本文： | 夏燕华,赵天生,张楚瑜. 猪瘟病毒全长突变型cDNA克隆的构建及其在细胞水平的恢复[J]. 南昌大学学报(理科版), 2009, 33(4): 1 |
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| 作者姓名： | 夏燕华 赵天生 张楚瑜 |
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| 作者单位： | 南昌大学医学院； 武汉大学生命科学学院； |
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| 基金项目： | 国家自然科学基金资助项目(30370055) |
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| 摘 要： | 通过序列比对发现猪瘟病毒的强毒株和疫苗株在毒力基因Erns中存在两个差异氨基酸,据此在猪瘟病毒标准强毒sh im en株全长感染性cDNA克隆的基础上,(1)将疫苗株的Erns基因置换sh im en株的相应片段,构建嵌合型全长cDNA克隆;(2)对Erns基因进行定点突变,使293位和311位的丝氨酸和酪
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| 关 键 词： | 猪瘟病毒 体外转录 全长cDNA 感染性 |
Construction and Recovery of Full-length cDNA Mutation of Classical Swine Fever Virus(CSFV) |
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| XIA Yan-hua,ZHAO Tian-sheng,ZHANG Chu-yu. Construction and Recovery of Full-length cDNA Mutation of Classical Swine Fever Virus(CSFV)[J]. Journal of Nanchang University(Natural Science), 2009, 33(4): 1 |
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| Authors: | XIA Yan-hua ZHAO Tian-sheng ZHANG Chu-yu |
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| Affiliation: | 1.Medical College;Nanchang University;Nanchang 330006;China;2.College of Life Science;Wuhan University;Wuhan 430072;China |
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| Abstract: | Two different amino acids were found to exist in virulent Erns gene between highly virulent and avirulent strains of CSFV by sequence comparing.To explore the importance of the two amino acids in virulence of CSFV,three full-length cDNA mutations were constructed on the base of infectious cDNA clone of CSFV shimen strain.In one mutation Erns gene of Shimen was substituted by the corresponding region of HCLV while in the other two serine and tyrosine in site 293 and 311 were mutated into aspartoyl and histid... |
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