Spectrophotometric and fluorimetric determinations of trichothecene mycotoxins with reagents for formaldehyde |
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Authors: | Toshihiro Kato Yoshihiro Asabe Masao Suzuki Shoji Takitani |
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Institution: | Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12, Ichigaya-funagawaramachi. Shinjuku-ku, Tokyo 162 Japan |
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Abstract: | Several trichothecene mycotoxins, such as fusarenone-X (F-X) and T-2 toxin (T-2), readily liberate formaldehyde on heating with sulfuric acid. Spectrophotometric and fluorimetric methods for the determination of trichothecenes with reagents for formaldehyde are therefore possible. F-X (or T-2) can be determined in the 50–1000 mg l-1 (or 50–1500 mg l-1) range by the chromotropic acid method, in the 30–1200 mg l-1 (or 50–2000 mg l-1) range by the phenyl J acid method, and in the 1.25–25 mg l-1 (or 2–40 mg l-1) range by the J acid method. Other trichothecenes, neosolaniol, nivalenol, tetraacetylnivalenol, diacetoxyscirpenol and HT-2 toxin, etc. also give positive reactions but trichothecin and dihydronivalenol do not. |
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