Amperometric determination of phosphatidyl choline in serum with use of immobilized phospholipase d and choline oxidase |
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| Authors: | Isao Karube Kenji Hara Ikuo Satoh Shuichi Suzuki |
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| Institution: | Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 227 Japan |
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| Abstract: | Phospholipase D (EC 3.1.4.4.) and choline oxidase (EC 1.1.99.1.) are immobilized together on a hydrophobic agarose gel and used to convert the phospholipid to betaine and hydrogen peroxide, which is measured amperometrically at + 0.60 V vs. SCE. The response time of the sensor is 2 min, and the calibration curve for 0–3 g l-1 of phosphatidyl choline is linear. Different methods of insolubilizing the enzymes are compared. |
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