| 高效液相色谱法测定中药润燥止痒胶囊中大黄素和大黄素甲醚 |
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| 引用本文： | 夏玉吉,郑丽会,鲍家科,许乾丽,茅向军,王珍珍.高效液相色谱法测定中药润燥止痒胶囊中大黄素和大黄素甲醚[J].理化检验(化学分册),2012(2):147-149. |
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| 作者姓名： | 夏玉吉 郑丽会 鲍家科 许乾丽 茅向军 王珍珍 |
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| 作者单位： | 贵州师范大学山地环境重点实验室;贵州省药品检验所;贵阳医学院药学院 |
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| 基金项目： | 国家药典委员会2009国家药品标准提高研究课题(179号项目) |
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| 摘 要： | 提出了高效液相色谱法测定润燥止痒胶囊中大黄素和大黄素甲醚含量的方法。样品经甲醇-盐酸(10+0.3)混合溶液加热提取水解30min,采用Hypersil ODS 2C18色谱柱为分离柱,以不同体积比混合的甲醇和磷酸(0.1+99.9)溶液为流动相进行梯度洗脱,检测波长为254nm。大黄素和大黄素甲醚的平均回收率分别为100.2%和99.7%。
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| 关 键 词： | 高效液相色谱法 润燥止痒胶囊 大黄素 大黄素甲醚 |
HPLC Determination of Emodin and Physcion in the TCM of "Runzao Zhiyang" Capsule |
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| XIA Yu-ji,ZHENG Li-hui,BAO Jia-ke,XU Qian-li,MAO Xiang-jun,WANG Zhen-zhen.HPLC Determination of Emodin and Physcion in the TCM of "Runzao Zhiyang" Capsule[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2012(2):147-149. |
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| Authors: | XIA Yu-ji ZHENG Li-hui BAO Jia-ke XU Qian-li MAO Xiang-jun WANG Zhen-zhen |
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| Institution: | 1.Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Nomoral Universtiy,Guiyang 550001,China;2.Guizhou Provincial Institute for Drug Testing, Guiyang 550004,China;3.Dept.of Pharmacy,Guiyang Medical College,Guiyang 550004,China) |
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| Abstract: | A method of HPLC for the determination of emodin and physcion in "Runzao Zhiyang" capsule was proposed.The sample was extracted with CH3OH-HCl(10+0.3) mixture and hydrolyzed for 30 min.The extracts were separated on Hypersil ODS 2 C18 column by gradient elution with mixtures of CH3OH and H3PO4-H2O(0.1+99.9) solution in different ratio,and determined at the wavelength of 254 nm.Values of average recovery found were 100.2% for emodin and 99.7% for physcion. |
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| Keywords: | HPLC "Runzao Zhiyang" capsule Emodin Physcion |
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