TLC as a pilot technique for the optimization of gradient HPLC II. Use of data obtained from RP-18 plates for the prediction of gradient programs in reversed-phase liquid chromatography |
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| Authors: | W Go?kiewicz |
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| Institution: | (1) Department of Inorganic and Analytical Chemistry, Institute of Basic Chemical Sciences of the Medical Academy, 20-081 Lublin, Poland |
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| Abstract: | Summary A method is described and experimentally verified permitting the determination of the optimal gradient programs in HPLC on the basis of isocratic TLC data. The proposed method has many advantages and can also be used in reversed-phase systems. A satisfactory agreement between the theoretical and experimental of VR/VO values has been confirmed. Some discrepancies for more strongly retained solutes are, probably due to the respective equilibrium and nonequilibrium state of the column in isocratic and gradient elution.Part I: Ref. 1]. |
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| Keywords: | Liquid chromatography Reversed-phase chromatography Gradient-elution chromatography Stepwise and continuous gradient Thin-layer chromatography |
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