红外光谱法与药用玫瑰真伪的分析与鉴定

文章采用红外光谱法(FTIR)并结合二维相关光谱分析技术分析鉴定了药用玫瑰(药材对照品)及其混淆品月季和蔷薇。在一维红外光谱中三者的谱图较为一致，但药用玫瑰在1 318 cm-1波数处的峰不明显 ，而月季和蔷薇的1 318 cm-1峰明显，且较尖。在高分辨的二阶导数谱中药用玫瑰和月季分别在1 617和1 618 cm-1处具有芳香类指纹特征峰，而蔷薇的峰则在1 612 cm-1波数处，相差了5～6 cm-1。在1 044 cm-1处的峰, 蔷薇糖类化合物的指纹特征峰较明显，月季和药用玫瑰不明显。观测二维相关红外谱，三者都存在1 620，1 560和1 460 cm-1的芳香类化合物的自动峰，不同的是月季和蔷薇还存在一个较明显的1 660 cm-1的肩膀峰。药用玫瑰、月季和蔷薇在850～1 250 cm-1范围内的二维相关红外光谱差别很大，药用玫瑰1 050 cm-1的糖类化合物自动峰强度最大，而月季的峰较弱，而蔷薇没有此峰。谱图的三级鉴定验证了药用玫瑰、月季和蔷薇在糖苷类化合物和芳香类化合物的相对含量上都是不一致的。结果表明，运用红外光谱法可以快速有效地分析和鉴定药用玫瑰及其混淆品。

Identification and Analysis of Genuine and False Flos Rosae Rugosae by FTIR and 2D Correlation IR Spectroscopy

The genuine and false Flos Rosae Rugosae (Flos Rosae Chinensis and Flos Rosa multiflora) were examined in terms of their differences by using Fourier transforrn infrared spectroscopy (FTIR) combined with two-dimensional (2D) correlation IR spectroscopy. The three species were shown very similar in FTIR spectra. The peak of 1 318 cm-1 of genuine Flos Rosae Rugosae is not obvious but this peak could be found sharp in Flos Rosae Chinensis and Flos Rosa multiflora. Generally, the second derivative IR spectrum can clearly enhance the spectral resolution. Flos Rosae Rugosae and Flos rosae Chinensis have aromatic compounds distinct fingerprint characteristics at 1 617 and 1 618 cm-1, respectively. Nevertheless, Flos Rosa multiflora has the peak at 1 612 cm-1. There is a discrepancy of 5 to 6 cm-1. Flos Rosa multiflora has glucide’s distinct fingerprint characteristics at 1 044 cm-1， but Flos Rosae Rugosae and Flos Rosae Chinensis don’t. The second derivative infrared spectra indicated different fingerprint characteristics. Three of them showed aromatic compounds with autopeaks at 1 620, 1 560 and 1 460 cm-1. Flos Rosae Chinensis and Flos Rosa multiflora have the shoulder peak at 1 660 cm-1. In the range of 850-1 250 cm-1, three of them are distinct different, Flos Rosae Rugosae has the strongest autopeak, Flos Rosae Chinensis has the feeble autopeak and Flos Rosa multiflora has no autopeak at 1 050 cm-1. In third-step identification, the different contents of aromatic compounds and glucide in Flos Rosae Rugosae, Flos Rosae Chinensis and Flos Rosa multiflora were revealed. It is proved that the method is fast and effective for distinguishing and analyzing genuine Flos Rosae Rugosae and false Flos Rosae Rugosae (Flos Rosae Chinensis and Flos Rosa multiflora).