Assuring the reliability of mass spectrometry for the routine determination of traces of doping in horse-urine |
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Authors: | I M H Lima M N Frota E A N Fernandes P Bode F S Tagliaferro |
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Institution: | 39701. Jockey Club Brasileiro, Av. Bartolomeu Mitre 1314, 22431-000, Rio de Janeiro, Brazil 39702. Pontifícia Universidade Católica, Rua Marqu?s de S?o Vicente 225, 22453-900, Rio de Janeiro, Brazil 39703. Centro de Energia Nuclear na Agricultura, University of S?o Paulo, 13400-970, PO Box 96, Piracicaba, SP, Brazil 39704. Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629JB, Delft, The Netherlands 39705. Centro de Energia Nuclear na Agricultura, University of S?o Paulo, 13400-970, PO Box 96, Piracicaba, SP, Brazil
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Abstract: | Summary This paper describes an analytical method using a nuclear-related technique for the detection of forbidden doping substances
in the urine of race horses. The proposed method, adapted from the Méthode Alcaline Sur C-18 developed by the French Laboratoire
de Contr?le Antidopage, is based on gas chromatography separation followed by mass spectrometry (GC-MS). The method was validated
for caffeine, identified as the most frequent doping substance in the Brazilian horseracing activity. This validation is also
a major requirement to achieve ISO/IEC 17025 laboratory accreditation. The validation has led to several metrological challenges
because the decisions are largely based on qualitative results (“false-positive” and/or “false-negative”) and the degree of
accuracy, as well as the traceability had to be determined in the absence of certified matrix reference materials. |
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