Separation of aminoacidato ruthenium(II) complexes by ion-pair chromatography

Summary The chromatographic conditions for analytical separation of (⁶-C₆H₆) ruthenium(II) complexes with amino acid and dipeptide ligands have been investigated in dependence on their molecular structure. Different chromatographic phases were tested and the efficiency parameters were determined by the reversed-phase ion-pair technique. For the enantiomeric species L-penicillaminato ruthenium(II) complexes cis- and trans-(⁶-C₆H₆)Ru(L-Pen)H_–1]₂Cl₂ and the reaction byproducts a complete chromatographic separation has been obtained using sodium dodecylsulphate as an ion-pair reagent. For the purification procedure of the L-penicillaminato ruthenium(II) complexes, in order to characterize the separated pure complexes by ¹H-NMR spectroscopy, a preparative separation has been carried out using the volatile trifluoroacetic acid as an ion-pair reagent.Dedicated to Professor Dr. Wilhelm Fresenius on the occassion of his 80th birthday