Potentiometric coulometry based on charge accumulation with a peroxidase/osmium polymer-immobilized electrode for sensitive determination of hydrogen peroxide |
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| Institution: | 1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan;2. Department of Chemistry, Konan University, 8-9-1 Okamoto, Higashi-Nada, Kobe, Hyogo 658-8501, Japan;1. Analytical Development Research, Eisai Product Creation Systems, Eisai Company Ltd., Kamisu, Ibaraki 314-0255, Japan;2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan;1. School of Materials Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;2. Information Center, Second Military Medical University, Shanghai 200433, China;3. Department of Mechanical Engineering, The Hong Kong Polytechnic University, Hong Kong, China;4. Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China |
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| Abstract: | The charge accumulation due to peroxidase (POD)-catalyzed reduction of H2O2 in a test solution (4 μL) by Os(II) in a POD/PVIOs(dmebpy)2Cl]-immobilized layer on an electrode (PVI = poly(1-vinylimidazole), dmebpy = 4,4′-dimethyl-2,2′-bipyridine) was monitored potentiometrically for the detection of H2O2. Before potentiometry, the Os(II)/Os(III) ratio of the modified electrode was controlled by pre-electrolysis at a given potential in a separated electrolysis cell. The redox potential of the Os polymer film in the test solution shifted to the positive side on the addition of H2O2 and reached a constant value due to the accumulation of Os(III) in the film. The total amount of the accumulated charge was determined from the area of the portion corresponding to the redox potential shift on a reversible cyclic voltammogram recorded separately. The low detection limit (5 pmol H2O2) was realized with 82–90% of the recovery percentage. |
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