Sperm morphology of Trichospilus diatraeae and Palmistichus elaeisis (Hymenoptera: Chalcidoidea: Eulophidae) |
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| Affiliation: | 1. Departamento de Biologia Geral, Universidade Federal de Viçosa-UFV, Minas Gerais, Brazil;2. Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto-UFOP, Minas Gerais, Brazil;3. Departamento de Biologia Celular, Universidade Estadual de Campinas-UNICAMP, São Paulo, Brazil;1. Department of the Sciences of Agriculture, Food and Environment, University of Foggia, 71100, Foggia, Italy;2. Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Universidade de São Paulo (USP), 13418900, Piracicaba, SP, Brazil;3. Entomology Department, Washington State University, Mount Vernon Northwestern WA Research & Extension Center, Mount Vernon, WA, 98273-4768, United States;4. Ottenbohlstrasse 12, 88690, Mühlhofen, Germany;5. Allgemeine und Systematische Zoologie, Zoologisches Institut und Museum, Universität Greifswald, 17489, Greifswald, Germany;1. Laboratory of Host-Pathogen Dynamics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA;1. Department of Medicine, Microbiology and Immunology, McGill University, 3775 University Street, Montréal, QC H3A 2B4, Canada;2. Infectious Diseases and Immunity in Global Heath Program, Research Institute of the McGill University Health Centre, 1001 Boulevard Décarie, Montréal, QC H4A 3J1, Canada;3. Vector Molecular Biology Section, NIH, 12735 Twinbrook Parkway, Rockville, MD 20878, USA;4. Faculty of Health Science, Selahaddin Eyyubi University, 215 Bağcılar Mah. Şanlıurfa Blv Otogar Karşısı, Diyarbakir, 21090 Bağlar, Turkey;1. Applied Zoology, TU Dresden, Zellescher Weg 20b, 01217, Dresden, Germany;2. Interfaculty Institute for Cell Biology, Animal Genetics, Auf der Morgenstelle 15, 72076 Tübingen, Germany;3. Max-Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307, Dresden, Germany;4. Rzeszow University, Department of Biochemistry and Cell Biology, ul. Zelwerowicza 4, 35-601 Rzeszów, Poland;5. Max-Planck Institute for Developmental Biology, Spemannstr. 35, 72076 Tübingen, Germany;6. iBV, Université Nice Sophia-Antipolis, Parc Valrose, 06108, Nice, France |
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| Abstract: | In this study, the sperm morphology of the parasitoids Trichospilus diatraeae and Palmistichus elaeisis (Eulophidae) was investigated using light and transmission electron microscopy. In the two species, the sperm are spiral along their entire length and measure about 130 μm and 195 μm in length, respectively. The head region consists of the acrosome and nucleus. The acrosome is composed of an acrosomal vesicle and, in P. elaeisis, a perforatorium. In both species, an extracellular layer in which several filaments are radiated covers the acrosome and the anterior nuclear region. The nuclei are filled with homogeneous and compact chromatin and measure about 50 μm in length in P. elaeisis and 20 μm in T. diatraeae. The flagellum consists of an axoneme with the 9 + 9 + 2 microtubule arrangement spiraled in a long helix, two mitochondrial derivatives coiling around the axoneme and, in P. elaeisis, two accessory bodies. In T. diatraeae were observed transverse striations throughout the central region of the axoneme, whereas the central pair of microtubules was rarely observed. In the final flagellar region in T. diatraeae, different from P. elaeisis, one mitochondrial derivative ends well before the other and both end before the axoneme. The sperm of these two species exhibit features that discriminate one species from each other, as well as characteristics suggest that Eulophidae is closely related to Trichogrammatidae and both of these families are more similar to Eurytomidae than Agaonidae. |
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| Keywords: | Spiraled sperm Ultrastructure Parasitoid Insect |
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