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Methods and Effects of Hongshan Cock Spermatozoa Cryopreservation
作者姓名:LIU Jianzhong~  ZHANG Yiling~ . College of Chemical Engineering and Technology  Wuhan University of Science and Technology  Wuhan  Hubei  China  . College of Animal Science and Technology  Northwestern University of Science and Technology in Agriculture and Forest  Yangling  Shannxi  China
作者单位:LIU Jianzhong~1,ZHANG Yiling~2 1. College of Chemical Engineering and Technology,Wuhan University of Science and Technology,Wuhan 430081,Hubei,China; 2. College of Animal Science and Technology,Northwestern University of Science and Technology in Agriculture and Forest,Yangling 712100,Shannxi,China
基金项目:国家新技术研究发展基金(863计划)
摘    要:0Introduction Afterthecarryingoutofopen doorpolicies,espe ciallyinthelatesttenyears,livestockandpoul try’sraisingbusinessdevelopedveryfastinChina,whichhelpeduscompletelyshrugofftheembarrassed situationofsevereshortagesofanimalproteininthepastlongperiodandgreatlyexcitedandencouraged us.Meanwhilewemustclearlyrealizethepotentialdangersbehindthepresentprosperities.Ourbreeds arebecominglessandless,manynativebreedswithlowperformancedisappearedcompletely,thetendency oftheirextinctionisincreasinginth…

关 键 词:公鸡  精子  低温贮藏  受精能力
文章编号:1007-1202(2006)02-0447-04
收稿时间:2005-03-19

Methods and effects of Hongshan cock spermatozoa cryopreservation
LIU Jianzhong,ZHANG Yiling . College of Chemical Engineering and Technology,Wuhan University of Science and Technology,Wuhan ,Hubei,China, . College of Animal Science and Technology,Northwestern University of Science and Technology in Agriculture and Forest,Yangling ,Shannxi,China.Methods and Effects of Hongshan Cock Spermatozoa Cryopreservation[J].Wuhan University Journal of Natural Sciences,2006,11(2):447-450.
Authors:Liu Jianzhong  Zhang Yiling
Institution:(1) College of Chemical Engineering and Technology, Wuhan University of Science and Technology, 430081 Wuhan, Hubei, China;(2) College of Animal Science and Technology, Northwestern, University of Science and Technology in Agriculture and Forest, 712100 Yangling, Shannxi, China
Abstract:The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied. The result shows that the average survival rate of spermatozoa in straws was 0.451±0.056, and 0.390±0.040 in pellets, the former was significantly higher than the later (n=20,p<0.01). The average acrosomic integrity rate of the sperms was 0.613±0.049 in straws, and 0.476±0.057 in pellets. The former was significantly higher than the later (n=20,p<0.05). The fertility rates (%) of sperms in pellets, straws and freshly collected semen, at the third day after artificial insemination (AI) were 85.32±2.32, 87.73±1.00 and 90.77±1.68, respectively. The fertility rates of the three type of semen at the fourth and fifth days were lower than the third day’s. Variance analysis shows that the fertilities of the spermatozoa in pellets and straws were significantly lower than freshly collected semen (n=3,p<0.01), and the fertilities of the spermatozoa in straws were significantly higher than those in pellets (n=3,p<0.01). Foundation item: Supported by the National High-Technology Development Program of China (2001 AA213081) Biography: Liu Jianzhong (1967-), male, Ph. D., research direction; animal biotechnology.
Keywords:Hongshan cock spermatozoa  cryopreservation  fertilities
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