白蛋白酸致变性的动态激光散斑研究

利用动态激光散斑的方法研究了白蛋白的酸致变性过程。首先，利用动态散斑理论模拟生成了动态散斑序列图；然后，利用模拟和实验散斑图的时间序列散斑图及其灰度共生矩阵对白蛋白的酸致变性过程进行了分析；接着，又利用灰度共生矩阵的惯性矩和不同阶段的四幅散斑图的平均对比度图对该过程进行了更深入的研究。理论模拟和实验研究结果表明，在白蛋白酸致变性过程中，胶体溶液中的蛋白质微粒的运动由剧烈逐渐变得缓慢；同时，微粒数量减少而微粒尺寸增加。研究表明，动态激光散斑方法是一种实时、快速、有效的研究白蛋白变性过程的新手段。

Investigation of the acid denaturation of the albumin by dynamic laser speckle methods

The process of the acid denaturation of the albumin was studied using dynamic laser speckle methods in this work. The dynamic speckle pattern sequences were generated using the dynamic speckle theory firstly. And then, the acid denaturation process of the albumin was analyzed using the time history speckle patterns (THSP) and its gray level co-occurrence matrix (GLCM) obtained from simulation and experiments. Furthermore, this denaturation process was investigated throughly using the inertia moment of the GLCM and the mean contrast methods. The theoretical simulation and the experiments results show that the protein particles movement changed from high activity to low activity gradually in the colloid solution during the denaturation process, meanwhile, the quantity of the protein particles decreased and its size increased. The dynamic laser speckle method is a novel real-time, quick and effective method to study the albumin denaturation.