Rapid Determination of Aristolochic Acids I and II in Some Medicinal Plants by High-Performance Liquid Chromatography |
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Authors: | Wei Li Runkai Li Tao Bo Huwei Liu Xuefeng Feng Shilin Hu |
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Institution: | (1) The Key Lab of Bioorganic Chemistry and Molecular Engineering, Ministry of EducationPeking University College of Chemistry and Molecular Engineering, Beijing, 100871, P.R. China;(2) China Academy of Traditional Chinese Medicine, Beijing, 100700, P.R. China |
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Abstract: | Aristolochic acids (AA) are toxic components of Aristolochia plants which result in diseases of the kidney such as urothelial cancer. It is, therefore, essential to monitor the amount of aristolochic acid in herbal medicines. In this study a reversed-phase high-performance liquid-chromatographic (HPLC) method has been developed for rapid determination of aristolochic acids I and II. Baseline separation was achieved within five minutes by use of an ODS C18 column with methanol–water, 60:40, as mobile phase. Two kinds of aristolochic acid were successfully determined in 31 herbal samples of Aristolochia fangchi Wu and Caulis Aristolochiae Manshuriensis. The results indicated that in most samples the aristolochic acid I content is much higher than that of aristolochic acid II. The two kinds of aristolochic acid were not detected in Aristolochia fangchi from the Guangdong region, so Aristolochia fangchi from this region is recommended for use in herbal remedies. |
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Keywords: | Column liquid chromatography Medicinal plants Aristolochia fangchi Wu Caulis Aristolochiae Manshuriensis Aristolochic acid |
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