MODIFYING FACTORS OF THE CELLULAR CONCENTRATION OF PHOTOLYASE MOLECULES IN Saccharomyces cerevisiae CELLS–I. EFFECTS OF TEMPERATURE AND LIGHT |
| |
Authors: | Atsushi Fukui Wolfgang Laskowski |
| |
Institution: | Institute of Biophysics, Free University Berlin, D-1000 Berlin 33, W. Germany |
| |
Abstract: | Abstract— The effects of temperature and light on the cellular concentration of photoreactivating enzyme (PRE) molecules in haploid Saccharomyces cerevisiae cells were investigated. (1) Temperature effect: The number of active PRE molecules per cell (NPRE) in cells grown at 37°C was about 13% of that grown at 23°C, although the amount of proteins per cell remained the same. (2) Light effect: NPRE in cells grown in light was about 2.8 times larger than that grown in the dark. The value of NPRE in cells grown in the light decreased more rapidly during holding in buffer in the dark than in the light. The NPRE decrease during holding in buffer in the dark was more rapid in cells grown in the light than grown in the dark. A comparable decrease was observed after holding in buffer in the presence of cycloheximide. (3) In cells harboring a plasmid containing the gene PHR1, NPRE was larger in cells grown at 23 than at 30°C. |
| |
Keywords: | |
|
|