| 酶法测定水中的尿素含量 |
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| 引用本文： | 柳畅先,徐立. 酶法测定水中的尿素含量[J]. 分析试验室, 2000, 19(5): 18-20 |
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| 作者姓名： | 柳畅先 徐立 |
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| 作者单位： | 中南民族学院化学系,武汉,430074 |
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| 基金项目： | 国家民族委员会自然科学基金!(九五重点项目) |
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| 摘 要： | 利用脲酶、谷氨酸脱氢酶偶联催化尿素水解的原理，通过测定还原型烟酰胺腺嘌呤二核苷酸吸光度变化率得出其酶促反应速度，对应不同的尿素浓度制得标准曲线，讨论了ｐＨ值和抑制剂对测定的影响，实测了水样中尿素的含量。
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| 关 键 词： | 尿素 酶法测定 水样 水质监测 光度法 |
Enzymatic Determination of Urea in Water Samples |
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| LIU Chang-xian,XU Li. Enzymatic Determination of Urea in Water Samples[J]. Chinese Journal of Analysis Laboratory, 2000, 19(5): 18-20 |
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| Authors: | LIU Chang-xian XU Li |
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| Abstract: | Coupled enzymes of urease and glutamic dehydrogenase were used to catalyze hydrolysis of urea.The rate of absorbance change of nicotinamide adenine dinucleotide,reduced form (NADH) was determined to obtain the velocity of the enzymatic reaction, and the workin curve was used to determine urea in samples. The effect of pH and inhibitor was discussed. |
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| Keywords: | Urea Enzymatic determination Water |
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