Structural and functional analysis of T7D promoter and its complex withE. coli RNA polymerase |
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| Authors: | O. N. Ozoline I. S. Masulis V. V. Chasov N. N. Demina S. G. Kamzolova |
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| Affiliation: | (1) Institute of Cell Biophysics, Russian Academy of Sciences, 142292 Pushchino Moscow region, Russian Federation |
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| Abstract: | The DNA fragment containing D promoter of the T7 bacteriophage and its complex with DNA-dependent RNA polymerase undergo a conformational transition at 30 °C, which is accompanied by an increase in the rate of phosphodiester bond synthesis.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 7, pp. 1339–1374, July, 1995.The present work was supported by the International Science Foundation (project No. RMY300) and the Russian Foundation for Basic Research (project No. 94-04-11306a). |
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| Keywords: | promoter paranemic DNA structure conformational transition interaction with RNA polymerase abortive reaction rate |
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