The study of terbium generated bacterirhodopsin |
| |
Authors: | Bing?Xu Yue?Zhang Email author" target="_blank">Kunsheng?HuEmail author |
| |
Institution: | (1) Chinese Academy of Sciences, Institute of Biophysics, 100101 Beijing, China |
| |
Abstract: | The localization of Terbium (Tb3+) cations binding to deionized bacteriorhodopsin (bR) has been studied by using spectroscopic methods. It was found that adding
Tb3+ cations to deionized bR affects the fluorescence lifetimes of tryptophan (Trp) in bR, the wavelength of fluorescence peak
shifts “blue” and the peak value of fluorescence decreases. It was also found that adding one Tb3+ cation to deionized bR can restore the purple state from its blue state obviously. The measurements of absorbance, fluorescence
and lifetime of fluorescence also show that when more than three Tb3+ cations are added, no further changes can be found. It is suggested that one Tb3+ specific binding site for the color-controlling is located on the exterior of the bR trimer structure to negatively charged
lipids near Trp-10 and Trp-12. Three Tb3+ cations binding per bR is needed for the regenerated bR. |
| |
Keywords: | cation binding fluorescence bacteriorhodopsin terbium |
本文献已被 SpringerLink 等数据库收录! |