Amber suppressors of Erwinia chrysanthemi |
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| Institution: | 1. University Hospital Heidelberg, Department of Gynecology and Obstetrics, Im Neuenheimer Feld 440, 69120 Heidelberg, Germany;2. University Hospital Munich, Department of Gynecology and Obstetrics, Molecular Biology Laboratory, Maistrasse 11, 80337 München, Germany;1. Department of Orthopedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3‑1‑1 Maidashi, Higashi‑ku, Fukuoka 812‑8582, Japan;2. Kyocera Medical Corporation, 3‑3‑31 Miyahara, Yodogawa‑ku, Osaka , 532‑0003, Osaka, Japan;3. Division of Renewable Energy Dynamics, Research Institute for Applied Mechanics, Kyushu University, Fukuoka, Japan |
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| Abstract: | Mutations trpl and thyA1, both of a polyauxotrophic derivative of the Erwinia chrysanthemi strain B374, were characterized as amber mutations with an Escherichia coli suppressor, supA1P2, which inserts a glutamine in response to UAG. Simultaneous reversion of both mutations allowed us to isolate amber suppressor mutants of E. chrysanthemi. These suppressors were tested with a set of amber mutants of bacteriophage Mu which had been previously characterized on E. coli. The two independently isolated suppressors behaved as supD and supE mutants, respectively, of E. coli. |
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