Three new eudesmane sesquiterpenes from Artemisia vestita

Three eudesmane sesquiterpene lactones, namely artemivestinolides A-C(1-3), along with three known 11-epimeric lactones(4-6), were isolated from the aerial parts of Artemisia vestita. Their structures were elucidated on the basis of extensive spectroscopic analyses(IR, HR-ESIMS, 1D NMR and 2D NMR), and the absolute configurations were determined by single-crystal X-ray diffraction(with copper radiation). Furthermore, in an in vitro assay, the three new compounds exhibited a moderate inhibition of the lipopolysaccharide(LPS)-induced nitric oxide(NO) production in BV-2 microglial cells.