Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system |
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| Authors: | Luo Yuan Gelsinger-Austin Paul J Watson Jonathan M Barbastathis George Barton Jennifer K Kostuk Raymond K |
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| Institution: | Department of Electrical and Computing Engineering, University of Arizona, Tucson, AZ 85721, USA. luo@email.arizona.edu |
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| Abstract: | A three-dimensional imaging system incorporating multiplexed holographic gratings to visualize fluorescence tissue structures is presented. Holographic gratings formed in volume recording materials such as a phenanthrenquinone poly(methyl methacrylate) photopolymer have narrowband angular and spectral transmittance filtering properties that enable obtaining spatial-spectral information within an object. We demonstrate this imaging system's ability to obtain multiple depth-resolved fluorescence images simultaneously. |
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