Exploring the Nature of Photo-Damage in Two-photon Excitation by Fluorescence Intensity Modulation |
| |
Authors: | Arijit Kumar De Debabrata Goswami |
| |
Institution: | (1) Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, UP-208016, India |
| |
Abstract: | We investigate the relative photo-damage effects during one- and two-photon excitations and demonstrate that there exist fundamental
differences in the damage induced by a high repetition rate laser as compared to that of a CW laser. This difference is evident
from the degree of enhanced fluorescence intensity achieved by blanking the excitation with an optical chopper. Such an enhancement
in fluorescence intensity provides better signal-to-noise ratio that could have immediate applications in multiphoton imaging
of live specimens. |
| |
Keywords: | Two-photon cross-section Photo-damage Fluorescence enhancement Multiphoton imaging |
本文献已被 PubMed SpringerLink 等数据库收录! |