Study on the interaction between diphenhydramine and erythrosin by absorption,fluorescence and resonance Rayleigh scattering spectra |
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作者单位: | TANG XiaoLing(School of Chemistry and Chemical Engineering, Southwest China University, Chongqing 400715, China) ;
LIU ZhongFang(School of Chemistry and Chemical Engineering, Southwest China University, Chongqing 400715, China) ;
LIU ShaoPu(School of Chemistry and Chemical Engineering, Southwest China University, Chongqing 400715, China) ;
HU XiaoLi (School of Chemistry and Chemical Engineering, Southwest China University, Chongqing 400715, China) ; |
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摘 要: | In pH 4.5 Britton-Robinson(BR)buffer solution,erythrosin(ET)can react with diphenhydramine(DP)to form a 1:1 ion-association complex,which not only results in the change of the absorption spectra,but also results in the great enhancement of resonance Rayleigh scattering(RRS)and the quenching of fluorescence.Furthermore,a new RRS spectrum will appear,and the maximum RRS wavelength was located at about 580 nm.In this work,the spectral characteristics of the absorption,fluorescence and RRS,the optimum conditions of the reaction and the properties of an analytical chemistry were inves- tigated.A sensitive,simple and new method for the determination of DP by using erythrosin as a probe has been developed.The detection limits for DP were 0.0020μg/mL for RRS method,0.088μg/mL for absorption method and 0.094μg/mL for fluorophotometry.There was a linear relationship between the absorbance,RRS and fluorescence intensities and the drug concentration in the range of 0.0067-2.0, 0.29-6.4 and 0.31-3.2μg/mL,respectively.The effects of the interaction of diphenhydramine and erythrosin on the absorption,fluorescence and resonance Rayleigh scattering spectra were discussed. In light polarization experiment,the polarization of RRS at maximum wavelength was measured to be P =0.9779,and it revealed that the RRS spectrum of DP-ET complex consists mostly of resonance scat- tering and few resonance fluorescence.In this study,enthalpy of formation and mean polarizability were calculated by AM1 quantum chemistry method.In addition,the reaction mechanism and the rea- sons for the enhancement of scattering spectra and the energy transfer between absorption,fluores- cence and RRS were discussed.
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收稿时间: | 26 April 2006 |
修稿时间: | 2 August 2006 |
Study on the interaction between diphenhydramine and erythrosin by absorption,fluorescence and resonance Rayleigh scattering spectra |
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Authors: | Tang XiaoLing Liu ZhongFang Liu ShaoPu Hu XiaoLi |
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Institution: | School of Chemistry and Chemical Engineering, Southwest China University, Chongqing 400715, China |
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Abstract: | In pH 4.5 Britton-Robinson (BR) buffer solution, erythrosin (ET) can react with diphenhydramine (DP) to form a 1:1 ion-association
complex, which not only results in the change of the absorption spectra, but also results in the great enhancement of resonance
Rayleigh scattering (RRS) and the quenching of fluorescence. Furthermore, a new RRS spectrum will appear, and the maximum
RRS wavelength was located at about 580 nm. In this work, the spectral characteristics of the absorption, fluorescence and
RRS, the optimum conditions of the reaction and the properties of an analytical chemistry were investigated. A sensitive,
simple and new method for the determination of DP by using erythrosin as a probe has been developed. The detection limits
for DP were 0.0020 μg/mL for RRS method, 0.088 μg/mL for absorption method and 0.094 μg/mL for fluorophotometry. There was
a linear relationship between the absorbance, RRS and fluorescence intensities and the drug concentration in the range of
0.0067–2.0, 0.29–6.4 and 0.31–3.2 μg/mL, respectively. The effects of the interaction of diphenhydramine and erythrosin on
the absorption, fluorescence and resonance Rayleigh scattering spectra were discussed. In light polarization experiment, the
polarization of RRS at maximum wavelength was measured to be P = 0.9779, and it revealed that the RRS spectrum of DP-ET complex consists mostly of resonance scattering and few resonance
fluorescence. In this study, enthalpy of formation and mean polarizability were calculated by AM1 quantum chemistry method.
In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra and the energy transfer between
absorption, fluorescence and RRS were discussed.
Supported by the National Natural Science Foundation of China (Grant No. 20475045) |
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Keywords: | resonance Rayleigh scattering spectrophotometry fluorescence quenching method diphenhydramine erythrosin |
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