| DGD包埋－去包埋样品的免疫标记 |
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| 引用本文： | 梁凤霞,丁明孝,翟中和.DGD包埋－去包埋样品的免疫标记[J].电子显微学报,1995(1). |
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| 作者姓名： | 梁凤霞 丁明孝 翟中和 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 小鼠膀胱上皮细胞经细胞选择性抽提和ＤＧＤ包埋后，制备超薄切片，并对其进行免疫标记。结果表明，ＤＧＤ包埋－去包理样品中的ＡＵＭ蛋白仍可特异地被抗体所识别，这一结果为证明小鼠膀胱上皮细胞中由ＡＵＭ蛋白组成的梭形泡以及腔面不对称单位膜结构均与中间纤维相连提供了证据。本文还对ＤＧＤ白埋后样品免疫标记技术进行了讨论。
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| 关 键 词： | 免疫标记技术，DGD包埋－去包埋技术，膀胱上皮，不对称单位膜 |
Immunolabelling for Ultrathin Sections of DGD Embedded Sample |
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| Liang Fengxia Ding Mingxiao Zhai Zhonghe.Immunolabelling for Ultrathin Sections of DGD Embedded Sample[J].Journal of Chinese Electron Microscopy Society,1995(1). |
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| Authors: | Liang Fengxia Ding Mingxiao Zhai Zhonghe |
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| Abstract: | Mouse bladder urothelial cell was selectively extracted and then embedded in DGD. The section of DGD sample was labeled with anti-AUM antibodies. The results show that AUM could be specifically labeled.It pre-sents a direct evidence to prove the connection of the intermediate filaments with the fusiform vesicles in the ep ithelial cell.The advantages and weakness of the technique are also discussed in this paper. |
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| Keywords: | immunolabelling technique DGD embedding and embedment-free technique bladder urothelium asymmetrical unit membrane(AUM) |
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