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Dynamic SIMS analysis of cryo-prepared biological and geological specimens |
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| Authors: | M Dickinson PJ Heard AC Lewis GC Allen |
| Institution: | a Interface Analysis Centre, University of Bristol, 121 St. Michael's Hill, Bristol BS2 8BS, UK b Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK c Department of Earth Sciences, University of Bristol, Wills Memorial Building, Bristol BS8 1RJ, UK |
| Abstract: | The modification of a dynamic magnetic sector secondary ion mass spectrometry (SIMS) instrument to permit the analysis of frozen biological and geological samples is described. The SIMS instrument used for this study combines SIMS analysis with the generation of ion-induced secondary electron images, allowing direct superposition of the SIMS image onto the image of cellular structures. Secondary ion maps have been acquired with sub-micron resolution, permitting the characterisation of sub-cellular elemental distributions in plant cells and human fibroblast cells, as well as the distribution of chemical impurities at grain boundaries in polar ice samples. This cryo-preparation technique clearly extends the applicability of SIMS analysis to a wide range of samples. |
| Keywords: | Cryo SIMS Biological Pteris vittata Fibroblast cells Polar ice |
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