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水稻基因表达谱分析寡核苷酸芯片制备的研究
引用本文:李援亚,曾黎琼,王力,张云孙.水稻基因表达谱分析寡核苷酸芯片制备的研究[J].云南大学学报(自然科学版),2007,29(5):515-518.
作者姓名:李援亚  曾黎琼  王力  张云孙
作者单位:1. 云南大学,生命科学学院,云南,昆明,650091
2. 云南省农业科学院,生物技术与种质资源研究所,云南,昆明,650223
3. 成都军区昆明总医院,云南,昆明,650032
4. 云南大学,科学技术处,云南,昆明,650091
基金项目:云南省自然科学基金(青年项目)资助项目(2005C0006Q,2003C0079M)
摘    要: 首先有目的地搜集了50个水稻花序相关基因,进行了探针的设计、筛选及合成纯化,用点样仪以微阵列的形式将其点于醛基化的玻璃片上;将3个不同生长阶段的水稻花序材料的总RNA经荧光标记反转录后与寡核苷酸芯片进行杂交.用ScanArray3000对获得的表达谱进行扫描分析显示,芯片图像背景均匀,信号清晰.用ImaGene4.0软件对表达谱分析表明,候选基因在水稻花序3个不同发育阶段的材料中,表达水平有显著差异.为进一步进行水稻寡核苷酸芯片的制备及应用奠定了基础.

关 键 词:寡核苷酸芯片  水稻花序相关基因  基因表达谱
文章编号:0258-7971(2007)05-0515-04
修稿时间:2007-01-15

Establishment of rice oligonucleotide microarray in rice inflorescence development
LI Yuan-ya,ZENG Li-qiong,WANG Li,ZHANG Yun-shun.Establishment of rice oligonucleotide microarray in rice inflorescence development[J].Journal of Yunnan University(Natural Sciences),2007,29(5):515-518.
Authors:LI Yuan-ya  ZENG Li-qiong  WANG Li  ZHANG Yun-shun
Institution:1. School of Life Sciences, Yunnan University, Kunming 650091, China;2. Research Institute of Biotechnology &; Genetic Germplasm, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;3. Kunming General Hospital of Chengdu Military Command, Kunming 650032, China;4. Department of Science and Technology, Yunnan University, Kunming 650091, China
Abstract:Fifty rice inflorescence related genes from Internet,references were chosen.After probes of the 50 genes were designed and screened by BLAST analysis in the Internet,they were synthesized,purified and micro-arrayed onto the microscope sliders.Total RNA from 3 kinds of rice inflorescence tissues was extracted and labeled by fluorescent dye.The labeled probes were then hybridized with the oligonucleotide arrays.Expression patterns of 50 genes from rice inflorescence in different development were obtained by detection using ScanArray3000.It showed analyzed pots with uniformity background and clear signal.The plots of the images were acquired after the acquired gene expression patterns were analyzed by ImaGene4.0 software.The scatter plots and scale maps show that there existed a significant difference in the expression of these candidate genes in rice inflorescences with different development phase.Then,a foundation for the preparation of rice oligonucleotide microarray and farther application was layed.
Keywords:oligonucleotide microarray  rice inflorescence genes  gene expression profile
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