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Visualization of red-ox proteins on the gold surface using enzymatic polypyrrole formation |
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| Authors: | Almira Ramanaviciene Asta Kausaite-Minkstimiene Yasemin Oztekin Geta Carac Jaroslav Voronovic Natalija German Arunas Ramanavicius |
| Institution: | 1. Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania 3. Faculty of Science, Department of Chemistry, Selcuk University, Konya, Turkey 4. Faculty of Science, Department of Chemistry, University Dunarea de Jos of Galati, Domneasca Street 47, 800008, Galati, Romania 2. Division of Immunotechnology, State Research Institute Center for Innovative Medicine, Zygimantu 9, 01102, Vilnius, Lithuania |
| Abstract: | We describe a new method for the visualization of the activity of red-ox proteins on a gold interface. Glucose oxidase was selected as a model system. Surfaces were modified by adhesion of glucose oxidase on (a) electrochemically cleaned gold; (b) gold films modified with gold nanoparticles, (c) a gold surface modified with self-assembled monolayer, and (d) covalent immobilization of protein on the gold surface modified with a self-assembled monolayer. The simple optical method for the visualization of enzyme on the surfaces is based on the enzymatic formation of polypyrrole. The activity of the enzyme was quantified via enzymatic formation of polypyrrole, which was detected and investigated by quartz microbalance and amperometric techniques. The experimental data suggest that the enzymatic formation of the polymer may serve as a method to indicate the adhesion of active redox enzyme on such surfaces. An optical method for the evaluation of activity and distribution of glucose oxidase on the different surfaces was described. The enzymatic synthesis of polypyrrole (black colour) was successfully applied for the visualization of active enzyme on the surfaces. |
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