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A novel anti-interference and pH-modulation device: application to enzyme-free glucose detection |
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| Authors: | Yuhua Su Rongzong Hu Weixiong Huang Kangkang Hu |
| Institution: | 1. The Key Laboratory of Analytical Science of Xiamen University, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China |
| Abstract: | We report on a novel anti-interference and pH-modulation device (herein after referred to as ??device??). It is based on electrodialysis and can continuously increase the pH value of the carrier solution and - at the same time - remove interfering analytical signals obtained for ascorbic acid (AA) and uric acid (UA). The ??device?? was coupled to the FIA-amperometric detection of glucose. The linear range is from 1???mol?L?1 to 0.4?mmol?L?1, with a sensitivity of 213???A?cm?2?mM?1 and a detection limit of 1???mol?L?1 at a signal-to-noise ratio of 3. The method was used to sucessfully determine glucose in serum. This study represents a novel technique for overcoming analytical interference and is expected to find applications in liquid chromatography, for example in on-line pH-modulation if different pH values are needed for separation and detection. As shown in the figure, a specific electrolytic current was applied between the two electrodes. Thus H2O in the cathode chamber was electrolyzed to produce H2 and OH-. Then the OH- moved through the anion exchange membrane and got into the packed column by electromigration, where it mixed with the carrier solution of Na2SO4. Meanwhile, the SO 4 2- of the carrier solution moved through the other anion exchange membrane and entered the anode chamber. Therefore, the carrier solution of Na2SO4 was partly converted into NaOH after passing through the ??device??. |
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