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Centri-voltammetry for biosensing systems: biocentri-voltammetric xanthine detection
Authors:Ulku Anik  Serdar ?evik
Institution:1. Faculty of Science, Chemistry Department, Mugla University, 48000, K?tekli Mugla, Turkiye
Abstract:Centri-voltammetry is a method that combines centrifugation and voltammetry. This method, developed by our group in 2003, yielded promising results when applied to trace analysis of metal ios. We demonstrate here the first application of centri-voltammetry to biosensing systems. A xanthine biosensor was constructed by immobilizing xanthine oxidase on a planar platinum electrode which then was placed at the bottom of a centri-voltammetric cell. The experimental parameters were optimized to give two linear ranges. The first is from 0.1 to 1???M, and the second from 5 to 50???M. The RSD is 3.4 (n?=?5). The biosensor was applied to the determination of xanthine in wine and in urine. Calculated recoveries are 101?±?0.61% (n?=?3) for wine samples, and 102?±?0.556% (n?=?3) for urine samples.
Figure
Centri-voltammetry is a method, which combines the advantages of centrifugation and voltammetry. In this work, the performance of biocentri-voltammetry for xanthine detection has been investigated. After the optimization studies and examination of analytical characteristics, developed centri-voltammetric biosensor was applied for xanthine detection in wine and in urine samples.
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