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Rapid detection of Staphylococcus aureus via a sensitive DNA hybridization assay based on a long-lifetime luminescent europium marker |
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| Authors: | Min Ruan Cheng-Gang Niu Guang-Ming Zeng Pin-Zhu Qin Xiao-Yu Wang Da-Wei Huang Jing Huang Chang-Zheng Fan |
| Affiliation: | 1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China 2. Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Hunan University, Changsha, 410082, China |
| Abstract: | A two-probe tandem nucleic acid hybridization assay for detection of Staphylococcus aureus is presented. It is based on a europium(III) complex as a marker that has a long fluorescence lifetime, high quantum yield and can be easily conjugated to an oligonucleotide signaling probe. The amino-modified capture probe was associated with the signaling probe to form a two-probe tandem DNA pattern that is complementary to the target DNA. The method was optimized in terms of hybridization temperature, hybridization time and washing time. This resulted in good specificity and sensitivity when detecting such bacteria in food samples. A europium complex as a long fluorescent lifetime marker was conjugated to an oligonucleotide. The amino-modified capture probe was associated with the signaling probe to form a two-probe tandem DNA pattern that is complementary to the target DNA. The results are shown that this method has good specificity and sensitivity. |
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