| 从水稻悬浮细胞获得原生质体再生植株 |
| |
| 引用本文： | 贾勇炯,陈放.从水稻悬浮细胞获得原生质体再生植株[J].四川大学学报(自然科学版),1994,31(3):382-385. |
| |
| 作者姓名： | 贾勇炯 陈放 |
| |
| 摘 要： | 以长香稻（Ｏｒｙｚａ ｓａｔｉｖａ Ｌ．）（糯稻）的幼嫩种子限为材料，在ＭＳ和Ｎ６基本培养基上诱导产生结构松散的愈伤组织，在ＡＡ培养基中进行液体振荡培养，悬浮细胞经酶解后获得了少在性较好的原生质体，试验证明，水稻悬浮细胞是分离原生质体较理想的材料，在附加２．４－Ｄｌｍｇ／Ｌ（以下单位同），ＫＴ０．２，谷氨酰胺８６７，天门冬胺酸２６６的ＭＳ琼脂糖培养基中，诱导出了大量愈伤组织；在含ＫＴ２，ＮＡＡ０．
|
| 关 键 词： | 水稻 悬浮细胞 原生质体 植株再生 |
PLANT REGENERATION FROMSUSPENSIONCULTURE CELL- DERIVED PROTOPLASTSINRICE |
| |
| Jia Yongjiong Chen Fang Li Ying He Xiaoli.PLANT REGENERATION FROMSUSPENSIONCULTURE CELL- DERIVED PROTOPLASTSINRICE[J].Journal of Sichuan University (Natural Science Edition),1994,31(3):382-385. |
| |
| Authors: | Jia Yongjiong Chen Fang Li Ying He Xiaoli |
| |
| Institution: | Department of Biology |
| |
| Abstract: | Loose ealli from tender roots of oryza sativa L. on MS and N_6 media are the better materinl for producing protoplasts. More protoplasts were isolated from callus-derived suspension culture cells in an agitated liquid AA medium. Calli from protoplasts were developed in MS agrose medium containing 2,4- Dlmg/L(The following unit is the same) , KT 0.2 ,NAA 0.2 , Glutamine 867,Asparagine 266. Plantlets were induced on N_6 medium containing KT 2 ,NAA0.2 ,ZT0.2,CH 1000,4%Coconut milk. Regenerated plants were successfully planted in soil. |
| |
| Keywords: | rice root suspension culture cell protoplast plant regeneration |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
