Synthetic surfaces for ribonuclease adsorption |
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Authors: | Smith Bryan D Soellner Matthew B Raines Ronald T |
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Affiliation: | Department of Biochemistry and Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. |
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Abstract: | Intact RNA and DNA are of central importance to biochemical research and biotechnology. The preservation of these nucleic acids requires the absence of nuclease activity. Here, radical-mediated polymerization of vinylsulfonate on resin and glass surfaces is shown to produce a high-density poly(vinylsulfonate) coating that sequesters ribonucleases from aqueous solutions quickly and completely. The adsorptive efficacy of this coating exceeds that of other known coatings by > or =10(7)-fold. Surfaces coated with poly(vinylsulfonate) could be used to maintain the integrity of ribonucleic acids in a variety of contexts. |
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